Bethyl Laboratories
Rabbit anti-MBD3 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00693
TargetMBD3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Methyl-CpG binding domain protein 3 (MBD3) is a subunit of the NuRD complex and plays a role in recruiting histone deacetylases and DNA methyltransferases to DNA for transcriptional repression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 241 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMBD3
Antigen Species
Human
Gene ID53615
Gene Symbol
MBD3
Gene Name
methyl-CpG binding domain protein 3
Uniprot IDO95983
Protein Name
Methyl-CpG-binding domain protein 3
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MBD3 immobilized on solid support.
The epitope recognized by IHC-00693 maps to a region between residue 265 and 291 of human methyl-CpG binding domain protein 3 using the numbering given in entry NP_003917.1 (GeneID 53615).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00693Open
Product DatasheetPDF · IHC-00693 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00693 · Lot 1 (current lot)Open
Services
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