Bethyl Laboratories
Rabbit anti-MCAK IHC Antibody Affinity Purified
Primary
Catalog #IHC-00502
TargetMCAK
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationIHC
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Product Description
Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. MCAK (Mitotic Centromere-Associated Kinesin) is the founding member of kinesin-13 proteins. There are 3 independent genes coding for kinesin-13 proteins kif2a, kif2b, and kif2c (MCAK). Instead of functioning as a translocator of microtubules MCAK is a depolymerizer that is primarily responsible for releasing improper microtubule-kinetochore attachments during cell division.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMCAK
Antigen Species
Mouse
Gene ID73804
Gene Symbol
Kif2c
Gene Name
kinesin family member 2C
Uniprot IDQ922S8
Protein Name
Kinesin-like protein KIF2C
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support.
The epitope recognized by IHC-00502 maps to a region between residue 50 and 100 of mouse Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_608301.2 (GeneID 73804 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00502Open
Product DatasheetPDF · IHC-00502 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00502 · Lot 1 (current lot)Open
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