Bethyl Laboratories
Rabbit anti-MCM2 Antibody Affinity Purified
Primary
Catalog #A300-191A
TargetMCM2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMCM2
Antigen Species
Human
Gene ID4171
Gene Symbol
MCM2
Gene Name
minichromosome maintenance complex component 2
Uniprot IDP49736
Protein Name
DNA replication licensing factor MCM2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MCM2 immobilized on solid support.
The epitope recognized by A300-191A maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-191A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-191A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-191AOpen
Citations / Publications
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