Bethyl Laboratories
Rabbit anti-Mdc1/Nfbd1 Antibody Affinity Purified
Primary
Catalog #A304-843A
TargetMdc1/Nfbd1
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationIP
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Product Description
Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 900 and 950
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMdc1/Nfbd1
Antigen Species
Human
Gene ID240087
Gene Symbol
MDC1
Gene Name
mediator of DNA damage checkpoint 1
Uniprot IDQ5PSV9
Protein Name
Mediator of DNA damage checkpoint protein 1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Mdc1/Nfbd1 immobilized on solid support.
The epitope recognized by A304-843A maps to a region between residue 900 to 950 of human Mdc1/Nfbd1 using the numbering given in entry Q5PSV9 (GeneID 240087).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-843A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-843A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-843AOpen
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