Bethyl Laboratories
Rabbit anti-MED12 Recombinant Monoclonal Antibody [BLR084G]
Monoclonal Antibodies
Catalog #A700-084
TargetMED12
HostRabbit
CloneBLR084G
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR084G
Host
Rabbit
Immunogen
Between 2127 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetMED12
Antigen Species
Human
Gene ID9968
Gene Symbol
MED12
Gene Name
mediator complex subunit 12
Uniprot IDQ93074
Protein Name
Mediator of RNA polymerase II transcription subunit 12
Alternate Names
activator-recruited cofactor 240 kDa component • ARC240 • CAG repeat protein 45 • CAGH45 • FGS1 • HOPA • human opposite paired • MED12S • Mediator complex subunit 12 • mediator of RNA polymerase II transcription subunit 12 • mediator of RNA polymerase II transcription, subunit 12 homolog • OHDOX • OKS • OPA1 • OPA-containing protein • putative mediator subunit 12 • thyroid hormone receptor-associated protein complex 230 kDa component • thyroid hormone receptor-associated protein, 230 kDa subunit • TNRC11 • TRAP230 • trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit) • trinucleotide repeat-containing gene 11 protein
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 2127 and the C-terminus (residue 2177) of human mediator complex subunit 12 using the numbering given in entry NP_005111.2 (Gene ID 9968).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A700-084 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-084 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-084 · Lot 200501Open
Safety Data SheetPDF · A700-084Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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