Bethyl Laboratories
Rabbit anti-MED15 Antibody Affinity Purified
Primary
Catalog #A302-422A
TargetMED15
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96RN5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetMED15
Antigen Species
Human
Gene ID51586
Gene Symbol
MED15
Gene Name
mediator complex subunit 15
Uniprot IDQ96RN5
Protein Name
Mediator of RNA polymerase II transcription subunit 15
Alternate Names
activator-recruited cofactor 105 kDa component • activator-recruited cofactor, 105-kD • ARC105 • CAG7A • CTG repeat protein 7a • CTG7A • Mediator complex subunit 15 • mediator of RNA polymerase II transcription subunit 15 • PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein • PC2 glutamine/Q-rich-associated protein • PC2-glutamine-rich-associated protein • PCQAP • Positive cofactor 2 glutamine/Q-rich-associated protein • positive cofactor 2, glutamine/Q-rich-associated protein • TIG1 • TIG-1 • TNRC7 • TPA inducible gene-1 • TPA inducible protein • TPA-inducible gene 1 protein • trinucleotide repeat containing 7 • trinucleotide repeat-containing gene 7 protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MED15 immobilized on solid support.
The epitope recognized by A302-422A maps to a region between residue 450 and 500 of human mediator complex subunit 15 using the numbering given in entry NP_001003891.1 (GeneID 51586).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-422A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-422A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-422AOpen
Citations / Publications
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