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Rabbit anti-MED15 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
MED15
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 450 and 500
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Ferret, White-tufted-ear marmoset, Panda, Monkey, African elephant, Pig, Naked mole rat, Rabbit, Small-eared galago, Chinese hamster, Guinea pig_10141, Little brown bat, Thirteen-lined ground squirrel, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse, Rat, Mouse
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to MED15 immobilized on solid support.

The epitope recognized by IHC-00680-T maps to a region between residue 450 and 500 of human mediator complex subunit 15 using the numbering given in entry NP_001003891.1 (GeneID 51586).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96RN5].

51586

MED15

mediator complex subunit 15

Q96RN5

Mediator of RNA polymerase II transcription subuni

Alternate Names

activator-recruited cofactor 105 kDa component, activator-recruited cofactor, 105-kD, ARC105, CAG7A, CTG repeat protein 7a, CTG7A, Mediator complex subunit 15, mediator of RNA polymerase II transcription subunit 15, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, PC2-glutamine-rich-associated protein, PCQAP, Positive cofactor 2 glutamine/Q-rich-associated protein, positive cofactor 2, glutamine/Q-rich-associated protein, TIG1, TIG-1, TNRC7, TPA inducible gene-1, TPA inducible protein, TPA-inducible gene 1 protein, trinucleotide repeat containing 7, trinucleotide repeat-containing gene 7 protein

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.