Bethyl Laboratories
Rabbit anti-MEK1 Antibody Affinity Purified
Primary
Catalog #A302-140A
TargetMEK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
MEK1 is an activator and kinase for MAP and ERK kinases. It is a serine/threonine kinase essential to the MAP kinase signal transduction pathway which is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development. MEK1 displays 85% identity with MEK2. Although MEK1 and MEK2 have been found to be functionally similar, they may serve non-redundant functions. Both are required for cellular proliferation and may play a role in tumorigenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 343 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMEK1
Antigen Species
Human
Gene ID5604
Gene Symbol
MAP2K1
Gene Name
mitogen-activated protein kinase kinase 1
Uniprot IDQ02750
Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MEK1 immobilized on solid support.
The epitope recognized by A302-140A maps to a region between residue 343 and 393 of human MAPK/ERK kinase 1 (Dual specificity mitogen-activated protein kinase kinase 1) using the numbering given in entry NP_002746.1 (GeneID 5604).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-140A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-140A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-140AOpen
Citations / Publications
Services
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