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Primary Antibodies  

Rabbit anti-MELK Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

MELK

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 525 and 575

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Maternal embryonic leucine zipper kinase (MELK) phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may be involved in the inhibition of spliceosome assembly during mitosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q14680].

9833

MELK

maternal embryonic leucine zipper kinase

Q14680

Maternal embryonic leucine zipper kinase

Alternate Names

hMELK; HPK38; maternal embryonic leucine zipper kinase; pEg3 kinase; protein kinase Eg3; protein kinase PK38; tyrosine-protein kinase MELK

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.