Bethyl Laboratories
Rabbit anti-MgcRacGAP Antibody Affinity Purified
Primary
Catalog #A302-798A
TargetMgcRacGAP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 16 hours and 26 minutes for shipment by today
Product Description
Male germ cell Rac GTPase-activating protein (MgcRacGAP) is essential for the early stages of embryogenesis and may play a role in the microtubule-dependent steps in cytokinesis. MgcRacGAP plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. It also is involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation [taken from the Universal Protein Resource (UniProt) www antibody.uniprot.org/uniprot/ Q9H0H5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMgcRacGAP
Antigen Species
Human
Gene ID29127
Gene Symbol
RACGAP1
Gene Name
Rac GTPase activating protein 1
Uniprot IDQ9H0H5
Protein Name
Rac GTPase-activating protein 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MgcRacGAP immobilized on solid support.
The epitope recognized by A302-798A maps to a region between residue 200 and 250 of human Rac GTPase-Activating Protein 1 using the numbering given in entry NP_001119575.1 (GeneID 29127).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-798A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-798A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-798AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1