Bethyl Laboratories
Rabbit anti-MGRN1 Antibody Affinity Purified
Primary
Catalog #A302-912A
TargetMGRN1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
MGRN1 (mahogunin RING finger protein 1) is an E3 ubiquitin-protein ligase that mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. MGRN1 plays a role in the regulation of endosome-to-lysosome trafficking. It impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production but does not promote MC1R ubiquitination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O60291].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetMGRN1
Antigen Species
Human
Gene ID23295
Gene Symbol
MGRN1
Gene Name
mahogunin ring finger 1
Uniprot IDO60291
Protein Name
E3 ubiquitin-protein ligase MGRN1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MGRN1 immobilized on solid support.
The epitope recognized by A302-912A maps to a region between residue 400 and 450 of human Mahogunin, Ring Finger 1 using the numbering given in entry NP_001135761.1 (GeneID 23295).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-912A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-912A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-912AOpen
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