Rabbit anti-MGRN1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
MGRN1 (mahogunin RING finger protein 1) is an E3 ubiquitin-protein ligase that mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. MGRN1 plays a role in the regulation of endosome-to-lysosome trafficking. It impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production but does not promote MC1R ubiquitination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O60291].
Alternate Names
E3 ubiquitin-protein ligase MGRN1; mahogunin ring finger 1, E3 ubiquitin protein ligase; mahogunin RING finger protein 1; probable E3 ubiquitin-protein ligase MGRN1; RING finger protein 156; RING-type E3 ubiquitin transferase MGRN1; RNF156
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.