Bethyl Laboratories
Rabbit anti-MID1 Antibody Affinity Purified
Primary
Catalog #A302-227A
TargetMID1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Defects in the Midline-1 (MID1) gene are the cause of Opitz syndrome, an X-linked recessive disorder characterized by developmental defects. MID1 is a microtubule-associated protein (MAP) with E3 ubiquitin ligase activity. MID1 has been shown to target protein phosphatases 2A (PP2Ac) on microtubules for degradation. MID1 association with PP2A has been proposed to regulate microtubule stabilization for cellular processes such as cell division and migration.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 617 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMID1
Antigen Species
Human
Gene ID4281
Gene Symbol
MID1
Gene Name
midline 1 (Opitz/BBB syndrome)
Uniprot IDO15344
Protein Name
E3 ubiquitin-protein ligase Midline-1
Alternate Names
BBBG1 • E3 ubiquitin-protein ligase Midline-1 • FXY • GBBB1 • MIDIN • midline 1 RING finger protein • OGS1 • Opitz/BBB syndrome • OS • OSX • putative transcription factor XPRF • RING finger protein 59 • RING finger protein Midline-1 • RING-type E3 ubiquitin transferase Midline-1 • RNF59 • TRIM18 • tripartite motif protein TRIM18 • tripartite motif-containing protein 18 • XPRF • zinc finger on X and Y, mouse, homolog of • ZNFXY
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MID1 immobilized on solid support.
The epitope recognized by A302-227A maps to a region between residue 617 and 667 of human midline 1 using the numbering given in entry NP_000372.1 (GeneID 4281).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-227A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-227A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-227AOpen
Citations / Publications
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