/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MIS12 Antibody
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Primary Antibodies  

Rabbit anti-MIS12 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

MIS12

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 150 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

MIS12 is an inner kinetochore protein found in a complex with Dsn1, Nnf1, and Nsl1 that functions in kinetochore assembly. This MIS12 complex has been demonstrated to be required for proper chromosome alignment and cell cycle progression.

79003

MIS12

MIS12, kinetochore complex component

Q9H081

Protein MIS12 homolog

Alternate Names

2510025F08Rik; hMis12; homolog of yeast Mis12; KNTC2AP; MIS12 homolog; MIS12, MIND kinetochore complex component, homolog; MTW1; protein MIS12 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.