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Rabbit anti-MKK4 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
MKK4
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 50 and 100
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to MKK4 immobilized on solid support.

The epitope recognized by A302-657A-T maps to a region between residue 50 and 100 of human dual specificity mitogen-activated protein kinase kinase 4 using the numbering given in entry NP_003001.1 (GeneID 6416).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

MAPKK4 (mitogen-activated protein kinase kinase 4) is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. MAPKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. Studies in knockout mice suggest that MAPKK4 mediates survival signals in T cell development. It may also be important to liver organogenesis.[taken from NCBI Entrez Gene (GeneID: 6416)].

6416

MAP2K4

mitogen-activated protein kinase kinase 4

P45985

Dual specificity mitogen-activated protein kinase

Alternate Names

c-Jun N-terminal kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK kinase 1, SAPK/ERK kinase 1, SAPKK1, SAPKK-1, SEK1, SERK1, SKK1, stress-activated protein kinase kinase 1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.