Bethyl Laboratories
Rabbit anti-MPI Antibody Affinity Purified
Primary
Catalog #A305-495A
TargetMPI
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants [taken from NCBI Entrez Gene (Gene ID: 4351)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMPI
Antigen Species
Human
Gene ID4351
Gene Symbol
MPI
Gene Name
mannose phosphate isomerase
Uniprot IDP34949
Protein Name
Mannose-6-phosphate isomerase
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MPI immobilized on solid support.
The epitope recognized by A305-495A maps to a region between residue 50 to 100 of human Mannose-6-phosphate isomerase using the numbering given in entry P34949.2 (GeneID 4351).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-495A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-495A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-495AOpen
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Resources
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