Bethyl Laboratories
Rabbit anti-MRCKalpha Antibody Affinity Purified
Primary
Catalog #A302-694A
TargetMRCKalpha
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
MRCKalpha (myotonic dystrophy kinase-related CDC42-binding kinase alpha) is a serine/threonine protein kinase that acts as a downstream effector of CDC42 and promotes actin reorganization.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1600 and 1650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMRCKalpha
Antigen Species
Human
Gene ID8476
Gene Symbol
CDC42BPA
Gene Name
CDC42 binding protein kinase alpha
Uniprot IDQ5VT25
Protein Name
Serine/threonine-protein kinase MRCK alpha
Alternate Names
CDC42 binding protein kinase alpha (DMPK-like) • CDC42 binding protein kinase beta • CDC42-binding protein kinase alpha • DMPK-like alpha • MRCK • MRCK alpha • MRCKA • Myotonic dystrophy kinase-related CDC42-binding kinase alpha • myotonic dystrophy kinase-related CDC42-binding protein kinase alpha • myotonic dystrophy protein kinase-like alpha • PK428 • serine/threonine-protein kinase MRCK alpha • ser-thr protein kinase PK428 • ser-thr protein kinase related to the myotonic dystrophy protein kinase
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MRCKalpha immobilized on solid support.
The epitope recognized by A302-694A maps to a region between residue 1610 and 1660 of human myotonic dystrophy protein kinase-like alpha using the numbering given in entry CAH71185.1 (GeneID 8476).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-694A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-694A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-694AOpen
Services
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Antibody and conjugate formats developed together, built to your specification.
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