Bethyl Laboratories
Rabbit anti-MSH2 Antibody Affinity Purified
Primary
Catalog #A300-451A
TargetMSH2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
MSH2 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. MSH2 has also been demonstrated to function as an anti-recombinase and may play a role in negatively regulating DNA repair. MSH2 can heterodimerize with MSH3 or MSH6. Mutations in MSH2 are linked to hereditary nonpolyposis colon cancer (HNPCC).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMSH2
Antigen Species
Human
Gene ID4436
Gene Symbol
MSH2
Gene Name
mutS homolog 2
Uniprot IDP43246
Protein Name
DNA mismatch repair protein Msh2
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MSH2 immobilized on solid support.
The epitope recognized by A300-451A maps to a region between residues 1 and 50 of human mutS homolog 2 using the numbering given in Swiss-Prot entry P43246 (GeneID 4436).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-451A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-451A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-451AOpen
Citations / Publications
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