Bethyl Laboratories
Rabbit anti-MST4/MASK Antibody Affinity Purified
Primary
Catalog #A300-565A
TargetMST4/MASK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
MST4/STK24 is a member of the sterile 20 (Ste20)-like kinase family that acts upstream of mitogen-activated protein kinase (MAPK) pathways and regulates c-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK). Ste20-like kinases are involved in a variety of cellular processes which include gene transcription, stress responses, cytoskeletal organization and apoptosis. In response to apoptotic signals, MST4/MASK is cleaved by caspase-3 and mediates apoptosis. MST4/MASK has been shown to influence cell growth and transformation by modulating ERK (extracellular signal-regulated kinase) signaling and may play a role in prostrate cancer. MST4/MASK may also play a role in cell motility.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMST4/MASK
Antigen Species
Human
Gene ID51765
Gene Symbol
STK26
Gene Name
serine/threonine protein kinase 26
Uniprot IDQ9P289
Protein Name
Serine/threonine-protein kinase 26
Alternate Names
mammalian Ste20-like protein kinase 4 • mammalian sterile 20-like 4 • MASK • Mst3 and SOK1-related kinase • MST4 • MST-4 • serine/threonine protein kinase 26 • serine/threonine protein kinase MST4 • serine/threonine-protein kinase 26 • serine/threonine-protein kinase MASK • STE20-like kinase 4 • STE20-like kinase MST4
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MST4/MASK immobilized on solid support.
The epitope recognized by A300-565A maps to a region between residues 300 and 350 of human Mammalian Ste20-like Kinase 4 (Mst3 and SOK1-related kinase) using the numbering given in entry NP_057626.2 (GeneID 51765).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-565A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-565A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-565AOpen
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