Bethyl Laboratories
Rabbit anti-MTA3 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00423
TargetMTA3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
MTA3 (metastasis-associated protein 3) is a component of the NuRD (nucleosome remodeling and histone deacetylation) complex. MTA3 is a member of a family of proteins (MTA1-3) differentially expressed in metastatic breast cancer cells. MTA3 functions as a repressor of the transcription factor Snail, a factor involved in epithelial to mesenchymal transitions. MTA3 expression is estrogen dependent in breast cancer cells. Studies show that ERalpha selectively bind the MTA3 promoter in an SP1-dependent fashion to stimulate MTA3 gene transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMTA3
Antigen Species
Human
Gene ID57504
Gene Symbol
MTA3
Gene Name
metastasis associated 1 family member 3
Uniprot IDQ9BTC8
Protein Name
Metastasis-associated protein MTA3
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MTA3 immobilized on solid support.
The epitope recognized by IHC-00423 maps to a region between residues 400 and 450 of human metastasis associated 3 using the numbering given in entry Swiss-Prot entry Q9BTC8 (GeneID 57504)
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00423Open
Product DatasheetPDF · IHC-00423 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00423 · Lot 1 (current lot)Open
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