Bethyl Laboratories
Rabbit anti-MYBBP1A Antibody Affinity Purified
Primary
Catalog #A301-327A
TargetMYBBP1A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Myb binding protein 1a (MYBBP1a) was originally isolated as a c-Myb interacting protein but has been found to associate with a number of transcription factors. MYBBP1a has been shown to activate or repress transcription through its interaction with transcriptional regulators such as AHR, jun, c-Myb, NF-kappaB, and PGC-1alpha.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMYBBP1A
Antigen Species
Human
Gene ID10514
Gene Symbol
MYBBP1A
Gene Name
MYB binding protein 1a
Uniprot IDQ9BQG0
Protein Name
Myb-binding protein 1A
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MYBBP1A immobilized on solid support.
The epitope recognized by A301-327A maps to a region between residue 450 and 500 of human MYB binding protein (P160) 1a using the numbering given in entry NP_055335.1 (GeneID 10514).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A301-327A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-327A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-327AOpen
Services
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