NEW!

Rabbit anti-Myc Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Target:
Myc Tag
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP, Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

HRP (0.1 mg)

$268.00 $348.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Rabbits were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Rabbits were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
Country of Origin
USA

Additional Product Information

The Myc epitope tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized c-myc epitope represents amino acid residues 410-419, EQKLISEEDL, of human c-myc.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).