Rabbit anti-Myosin-9 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-490A-T maps to a region between residue 1900 to 1950 of human Myosin Heavy Chain 9 using the numbering given in entry NP_002464.1 (GeneID 4627). Antibody was affinity purified using an epitope specific to Myosin-9 immobilized on solid support.
The epitope recognized by A304-490A maps to a region between residue 1900 to 1950 of human Myosin Heavy Chain 9 using the numbering given in entry NP_002464.1 (GeneID 4627).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Mysosin-9 (MYH9) is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, mysosin 9 plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P35579].
Alternate Names
BDPLT6; cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MHA; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; myosin, heavy chain 9, non-muscle; myosin-9; NMHC-II-A; NMMHC II-a; NMMHCA; NMMHC-A; NMMHC-IIA; non-muscle myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; non-muscle myosin heavy polypeptide 9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.