Rabbit anti-MYPT1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
MYPT1 is the non-catalytic regulatory subunit of the myosin phosphatase (MP). MYPT1 is the smooth-muscle/platelet isoform. MYPT1 is a substrate of and is regulated by Rho-protein kinase. Phosphorylation of MYPT1 by Rho kinase inhibits MP activity.
Alternate Names
M130; MBS; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; MYPT1; protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.