/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MYPT1 Antibody
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Primary Antibodies  

Rabbit anti-MYPT1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

MYPT1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 800 and 850

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

MYPT1 is the non-catalytic regulatory subunit of the myosin phosphatase (MP). MYPT1 is the smooth-muscle/platelet isoform. MYPT1 is a substrate of and is regulated by Rho-protein kinase. Phosphorylation of MYPT1 by Rho kinase inhibits MP activity.

4659

PPP1R12A

protein phosphatase 1 regulatory subunit 12A

O14974

Protein phosphatase 1 regulatory subunit 12A

Alternate Names

M130; MBS; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; MYPT1; protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.