/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-N-Ras Recombinant Monoclonal Antibody [BLR326M]
Primary Antibodies  

Rabbit anti-N-Ras Recombinant Monoclonal Antibody [BLR326M]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

N-Ras

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR326M

Format:

Whole IgG

Immunogen:

between 50 and 100

Isotype:

IgG

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
500 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 50 and 100 of human GTPase N-Ras using the numbering given in entry NP_002515.1 (Gene ID 4893).

Additional Product Information

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. N-Ras plays an important role in the regulation of cell proliferation and plays a role in promoting oncogenic events and mutations in N-Ras have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [taken from NCBI Entrez Gene (Gene ID: 4893)].

4893

NRAS

NRAS proto-oncogene, GTPase

P01111

GTPase NRas

Alternate Names

ALPS4;CMNS;GTPase NRas;NCMS;neuroblastoma RAS viral (v-ras) oncogene homolog;neuroblastoma RAS viral oncogene homolog;N-ras;N-ras protein part 4;NRAS1;NS6;transforming protein N-Ras;v-ras neuroblastoma RAS viral oncogene homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.