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Primary Antibodies  

Rabbit anti-NAF1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

NAF1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 50 and 100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

H/ACA ribonucleoprotein complex non-core subunit NAF1 (nuclear assembly factor 1) is an RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. NAF1 probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96HR8].

92345

NAF1

nuclear assembly factor 1 ribonucleoprotein

Q96HR8

H/ACA ribonucleoprotein complex non-core subunit NAF1

Alternate Names

H/ACA ribonucleoprotein complex non-core subunit NAF1; hNAF1; nuclear assembly factor 1 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.