Bethyl Laboratories

Rabbit anti-NCBP1/CBP80 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00660
TargetNCBP1/CBP80
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Detection of human NCBP1/CBP80 by immunohistochemistry.
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Product Description

Nuclear cap binding protein subunit 1 (NCBP1) is part of the cap-bnding protein complex that associates with the 5’ cap structure of mRNAs to facilitate transcript stability, splicing efficiency, and 3’end formation.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
Antigen Species
Human
Gene ID4686
Gene Symbol
NCBP1
Gene Name
nuclear cap binding protein subunit 1
Uniprot IDQ09161
Protein Name
Nuclear cap-binding protein subunit 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to NCBP1/CBP80 immobilized on solid support.

The epitope recognized by IHC-00660 maps to a region between residue 482 and 532 of human nuclear cap binding protein subunit 1 (80 kDa nuclear cap-binding protein) using the numbering given in entry NP_002477.1 (GeneID 4686).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00660Open
Product DatasheetPDF · IHC-00660 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00660 · Lot 1 (current lot)Open
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