Bethyl Laboratories
Rabbit anti-NCBP2 Antibody Affinity Purified
Primary
Catalog #A302-553A
TargetNCBP2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Nuclear cap binding protein subunit 2 (NCBP2) is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP2 has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay [taken from NCBI Entrez Gene (GeneID: 22916)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 106 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNCBP2
Antigen Species
Human
Gene ID22916
Gene Symbol
NCBP2
Gene Name
nuclear cap binding protein subunit 2
Uniprot IDP52298
Protein Name
Nuclear cap-binding protein subunit 2
Alternate Names
20 kDa nuclear cap-binding protein • CBC2 • CBP20 • cell proliferation-inducing gene 55 protein • NCBP 20 kDa subunit • NCBP interacting protein 1 • NCBP-interacting protein 1 • NIP1 • nuclear cap binding protein subunit 2, 20kD • nuclear cap binding protein subunit 2, 20kDa • nuclear cap-binding protein subunit 2 • PIG55
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NCBP2 immobilized on solid support.
The epitope recognized by A302-553A maps to a region between residue 106 to 156 of human nuclear cap binding protein subunit 2 using the numbering given in entry NP_031388.2 (GeneID 22916).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-553A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-553A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-553AOpen
Citations / Publications
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