Bethyl Laboratories
Rabbit anti-NDE1 Antibody Affinity Purified
Primary
Catalog #A301-974A
TargetNDE1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
NDE1 (nuclear distribution factor E homolog 1) is important to microtubule organization, mitosis, and cell migration. It is required for the duplication of centrosomes and the function of the mitotic spindle. NDE1 is a binding partner of DISC1 (disrupted-in-schizophrenia 1) along with LIS1 (lissencephaly 1) at the centrosome and at synapses in neurons. It is also part of a multiprotein complex that regulates dynein. The NDE1 gene has been identified as a schizophrenia-associated locus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 285 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNDE1
Antigen Species
Human
Gene ID54820
Gene Symbol
NDE1
Gene Name
nudE neurodevelopment protein 1
Uniprot IDQ9NXR1
Protein Name
Nuclear distribution protein nudE homolog 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NDE1 immobilized on solid support.
The epitope recognized by A301-974A maps to a region between residue 275 and 325 of human nuclear distribution gene E using the numbering given in entry NP_060138.1 (GeneID 54820).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-974A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-974A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-974AOpen
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