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Rabbit anti-NEDD4 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
NEDD4
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 725 and 775
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to NEDD4 immobilized on solid support.

The epitope recognized by A303-253A-T maps to a region between residue 725 and 775 of human Neural Precursor Cell Expressed, Developmentally Down-regulated 4 using the numbering given in entry AAI36606.1 (GeneID 4734).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

NEDD4 (neural precursor cell expressed developmentally down-regulated protein 4) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Independent of its ubiquitin-ligase activity, NEDD4 is involved in the pathway leading to the degradation of VEGFR-2/KDFR [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P46934].

4734

NEDD4

neural precursor cell expressed, developmentally d

P46934

E3 ubiquitin-protein ligase NEDD4

Alternate Names

cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, HECT-type E3 ubiquitin transferase NEDD4, NEDD-4, NEDD4-1, Neural precursor cell expressed developmentally down-regulated protein 4, receptor-potentiating factor 1, RPF1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE.