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Primary Antibodies  

Rabbit anti-NEDD4 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NEDD4

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 725 and 775

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

NEDD4 (neural precursor cell expressed developmentally down-regulated protein 4) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Independent of its ubiquitin-ligase activity, NEDD4 is involved in the pathway leading to the degradation of VEGFR-2/KDFR [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P46934].

4734

NEDD4

neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase

P46934

E3 ubiquitin-protein ligase NEDD4

Alternate Names

cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; NEDD4-1; Neural precursor cell expressed developmentally down-regulated protein 4; receptor-potentiating factor 1; RPF1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.