Bethyl Laboratories
Rabbit anti-NEK1 Antibody Affinity Purified
Primary
Catalog #A304-570A
TargetNEK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Never in mitosis A-related kinase 1 (NEK1) is a serine/threonine kinase involved in cell cycle regulation. NEK1 is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD) [taken from NCBI Entrez Gene (Gene ID: 4750)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1208 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNEK1
Antigen Species
Human
Gene ID4750
Gene Symbol
NEK1
Gene Name
NIMA related kinase 1
Uniprot IDQ96PY6
Protein Name
Serine/threonine-protein kinase Nek1
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NEK1 immobilized on solid support.
The epitope recognized by A304-570A maps to a region between residue 1208 to 1258 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1 (GeneID 4750).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-570A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-570A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-570AOpen
Citations / Publications
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