Bethyl Laboratories
Rabbit anti-NELFA IHC Antibody Affinity Purified
Primary
Catalog #IHC-00383
TargetNELFA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
NELFA (negative elongation factor A) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELFA may play a role in Wolf-Hirschhorn syndrome (WHS).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 478 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetNELFA
Antigen Species
Human
Gene ID7469
Gene Symbol
NELFA
Gene Name
negative elongation factor complex member A
Uniprot IDQ9H3P2
Protein Name
Negative elongation factor A
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NELFA immobilized on solid support.
The epitope recognized by IHC-00383 maps to a region between residue 499 and 549 of human negative elongation factor A using the numbering given in entry BAB18651.1 (GeneID 7469).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00383Open
Product DatasheetPDF · IHC-00383 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00383 · Lot 1 (current lot)Open
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