Bethyl Laboratories
Rabbit anti-NELFE IHC Antibody Affinity Purified
Primary
Catalog #IHC-00385
TargetNELFE
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
NELFE (negative elongation factor E) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELF-E is an RNA-binding protein. The RNA binding activity of NELF-E is critical to the function of NELF.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 330 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetNELFE
Antigen Species
Human
Gene ID7936
Gene Symbol
NELFE
Gene Name
negative elongation factor complex member E
Uniprot IDP18615
Protein Name
Negative elongation factor E
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NELFE immobilized on solid support.
The epitope recognized by IHC-00385 maps to a region between residue 330 and 380 of human negative elongation factor polypeptide E using the numbering given in entry NP_002895.3 (GeneID 7936).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00385Open
Product DatasheetPDF · IHC-00385 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00385 · Lot 1 (current lot)Open
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