Bethyl Laboratories
Rabbit anti-NF-YA IHC Antibody Affinity Purified
Primary
Catalog #IHC-00576
TargetNF-YA
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 297 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetNF-YA
Antigen Species
Human
Gene ID4800
Gene Symbol
NFYA
Gene Name
nuclear transcription factor Y subunit alpha
Uniprot IDP23511
Protein Name
Nuclear transcription factor Y subunit alpha
Alternate Names
CAAT box DNA-binding protein subunit A • CAAT-box DNA binding protein subunit A • CBF-A • CBF-B • CCAAT-binding transcription factor subunit B • HAP2 • HAP2 CCAAT-binding protein • NF-YA • nuclear transcription factor Y subunit A • nuclear transcription factor Y subunit alpha • nuclear transcription factor Y, alpha • Transcription factor NF-Y, A subunit
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NF-YA immobilized on solid support.
The epitope recognized by IHC-00576 maps to a region between residue 333 and 347 of human nuclear transcription factor Y, alpha using the numbering given in entry NP_002496.1 (GeneID 4800).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00576Open
Product DatasheetPDF · IHC-00576 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00576 · Lot 1 (current lot)Open
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