Bethyl Laboratories
Rabbit anti-NF90 Antibody Affinity Purified
Primary
Catalog #A303-651A
TargetNF90
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
NFAR (nuclear factor associated with dsRNA) exists as two major protein species of 90 kDa (NFAR-1 or NF90) and 110 kDa (NFAR-2 or NF110) in the cell. NF90 and NF110 arise from a single gene named interleukin enhancer binding factor (ILF3) gene which resides on chromosome 19p13 and are generated through alternative splicing events. NF90 is a dsRNA binding protein that stabilizes mRNAs and regulates transcription. NF90 is a subunit of the nuclear factor of activated T-cells (NFAT) transcription factor that is required for T-cell expression of interleukin 2. The NF110 isoform is restricted to the nucleus and its function may be unrelated to NF90.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 675 and 725 of isoform 2
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNF90
Antigen Species
Human
Gene ID3609
Gene Symbol
ILF3
Gene Name
interleukin enhancer binding factor 3
Uniprot IDQ12906
Protein Name
Interleukin enhancer-binding factor 3
Alternate Names
CBTF • Double-stranded RNA-binding protein 76 • double-stranded RNA-binding protein, 76 kD • DRBF • DRBP76 • dsRNA binding protein NFAR-2/MPP4 • interleukin enhancer binding factor 3, 90kD • interleukin enhancer binding factor 3, 90kDa • interleukin enhancer-binding factor 3 • MMP4 • M-phase phosphoprotein 4 • MPHOSPH4 • MPP4 • NF110 • NF110b • NF90 • NF90a • NF90b • NFAR • NFAR-1 • NFAR2 • NF-AT-90 • nuclear factor associated with dsRNA • nuclear factor of activated T-cells 90 kDa • nuclear factor of activated T-cells, 90 kD • TCP110 • TCP80 • translational control protein 80
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NF90 immobilized on solid support.
The epitope recognized by A303-651A maps to a region between residue 652 and 702 of human Nuclear Factor of Activated T-cells, 90 kD using the numbering given in entry NP_004507.2 (GeneID 3609).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-651A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-651A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-651AOpen
Citations / Publications
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