Bethyl Laboratories
Rabbit anti-NFRKB Antibody Affinity Purified
Primary
Catalog #A301-459A
TargetNFRKB
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1025 and 1075
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNFRKB
Antigen Species
Human
Gene ID4798
Gene Symbol
NFRKB
Gene Name
nuclear factor related to kappaB binding protein
Uniprot IDQ6P4R8
Protein Name
Nuclear factor related to kappa-B-binding protein
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NFRKB immobilized on solid support.
The epitope recognized by A301-459A maps to a region between residue 1025 and 1075 of human nuclear factor related to kappaB binding protein using the numbering given in entry AAH63280.1 (GeneID 4798).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-459A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-459A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-459AOpen
Citations / Publications
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