Bethyl Laboratories
Rabbit anti-NOL1 Antibody Affinity Purified
Primary
Catalog #A302-018A
TargetNOL1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Nucleolar protein 1 (NOL1) is a protein highly expressed in proliferating cells; it is expressed in G1 and peaks during early S phase. It is proposed to play a role in the regulation of the cell cycle, and it may function as a ribosomal RNA methyltransferase.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 762 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNOL1
Antigen Species
Human
Gene ID4839
Gene Symbol
NOP2
Gene Name
NOP2 nucleolar protein
Uniprot IDP46087
Protein Name
Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
Alternate Names
NOL1 • NOL1/NOP2/Sun domain family, member 1 • NOP120 • NOP2 nucleolar protein homolog • NSUN1 • Nucleolar protein 1 • nucleolar protein 1, 120kDa • nucleolar protein 2 homolog • p120 • probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase • proliferating-cell nucleolar antigen p120 • proliferation-associated nucleolar protein p120 • putative ribosomal RNA methyltransferase NOP2
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NOL1 immobilized on solid support.
The epitope recognized by A302-018A maps to a region between residue 762 and 812 of human nucleolar protein 1 using the numbering given in entry AAH00656.1 (GeneID 4839).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-018A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-018A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-018AOpen
Citations / Publications
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