Bethyl Laboratories
Rabbit anti-NOPP140 Antibody Affinity Purified
Primary
Catalog #A302-184A
TargetNOPP140
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
NOPP140 (140 kDa nucleolar phosphoprotein) is implicated to play a role in ribosome assembly and the regulation of rRNA transcription. It has been observed to shuttle between the nucleus and cytoplasm and may also be important to nucleolar structural organization.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNOPP140
Antigen Species
Human
Gene ID9221
Gene Symbol
NOLC1
Gene Name
nucleolar and coiled-body phosphoprotein 1
Uniprot IDQ14978
Protein Name
Nucleolar and coiled-body phosphoprotein 1
Alternate Names
140 kDa nucleolar phosphoprotein • HCV NS5A trans-regulated protein 13 • HCV NS5A-transactivated protein 13 • hepatitis C virus NS5A-transactivated protein 13 • NOPP130 • NOPP140 • NS5ATP13 • nucleolar 130 kDa protein • nucleolar and coiled-body phosphoprotein 1 • nucleolar and coiled-body phosphprotein 1 • nucleolar phosphoprotein p130 • nucleolar protein p130 • P130
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NOPP140 immobilized on solid support.
The epitope recognized by A302-184A maps to a region between residue 1 and 50 of human nucleolar and coiled-body phosphoprotein 1 using the numbering given in entry NP_004732.2 (GeneID 9221).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-184A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-184A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-184AOpen
Citations / Publications
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