Bethyl Laboratories
Rabbit anti-Notch1 Antibody Affinity Purified
Primary
Catalog #A301-894A
TargetNotch1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 20 hours and 41 minutes for shipment by Tuesday, November 17
Product Description
Notch1 is the human homolog of the D. melanogaster Notch gene first reported as the gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch protein is a transmembrane receptor that mediates juxtacrine signals between adjacent cells. Mammalian Notch1 ligands include Delta-like and Jagged. Upon ligand binding, Notch1 undergoes two proteolytic cleavage events that result in the release of the intracellular Notch1 domain and its translocation to the nucleus where it can participate in gene transactivation. Notch 1 signals mediate cellular fate, differentiation, and development.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2425 and 2475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNotch1
Antigen Species
Human
Gene ID4851
Gene Symbol
NOTCH1
Gene Name
notch 1
Uniprot IDP46531
Protein Name
Neurogenic locus notch homolog protein 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Notch1 immobilized on solid support.
The epitope recognized by A301-894A maps to a region between residue 2400 and 2450 of human Notch ortholog 1, translocation-associated using the numbering given in entry NP_060087.3 (GeneID 4851).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-894A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-894A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-894AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1