Bethyl Laboratories
Rabbit anti-Notch1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00538
TargetNotch1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Notch1 is the human homolog of the D. melanogaster Notch gene first reported as the gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch protein is a transmembrane receptor that mediates juxtacrine signals between adjacent cells. Mammalian Notch1 ligands include Delta-like and Jagged. Upon ligand binding, Notch1 undergoes two proteolytic cleavage events that result in the release of the intracellular Notch1 domain and its translocation to the nucleus where it can participate in gene transactivation. Notch 1 signals mediate cellular fate, differentiation, and development.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2505 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetNotch1
Antigen Species
Human
Gene ID4851
Gene Symbol
NOTCH1
Gene Name
notch 1
Uniprot IDP46531
Protein Name
Neurogenic locus notch homolog protein 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Notch1 immobilized on solid support.
The epitope recognized by IHC-00538 maps to a region between residue 2505 and 2555 of human Notch ortholog 1, translocation-associated using the numbering given in entry NP_060087.3 (GeneID 4851).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00538Open
Product DatasheetPDF · IHC-00538 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00538 · Lot 1 (current lot)Open
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