/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Notch2 Antibody
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Primary Antibodies  

Rabbit anti-Notch2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

Notch2

Reactivity:

Human

Applications:

Flow Cyt

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 2275 and 2325

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Notch2 immobilized on solid support.

The epitope recognized by A302-083A-T maps to a region between residue 2250 and 2300 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).

Antibody was affinity purified using an epitope specific to Notch2 immobilized on solid support.

The epitope recognized by A302-083A maps to a region between residue 2250 and 2300 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

Notch2 is a member of the Notch family of transmembrane receptors. The Notch gene was first reported as a drosophila gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch proteins mediate juxtacrine signals between adjacent cells and regulate cell fate determination during development. In mammals there are 4 Notch receptors (Notch 1-4). Like Notch1, Notch 2 is required for embryo viability. Although Notch1 and Notch 2 are functionally similar, they may not serve redundant functions in the cell.

4853

NOTCH2

notch 2

Q04721

Neurogenic locus notch homolog protein 2

Alternate Names

AGS2; HJCYS; hN2; neurogenic locus notch homolog protein 2; Notch 2; Notch homolog 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.