Bethyl Laboratories
Rabbit anti-Notch2 Antibody Affinity Purified
Primary
Catalog #A302-084A
TargetNotch2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationFlow Cyt, IHC, IP, WB
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Product Description
Notch2 is a member of the Notch family of transmembrane receptors. The Notch gene was first reported as a drosophila gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch proteins mediate juxtacrine signals between adjacent cells and regulate cell fate determination during development. In mammals there are 4 Notch receptors (Notch 1-4). Like Notch1, Notch 2 is required for embryo viability. Although Notch1 and Notch 2 are functionally similar, they may not serve redundant functions in the cell.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2421 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNotch2
Antigen Species
Human
Gene ID4853
Gene Symbol
NOTCH2
Gene Name
notch 2
Uniprot IDQ04721
Protein Name
Neurogenic locus notch homolog protein 2
Alternate Names
Product Information
Applications
Flow Cyt, IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Notch2 immobilized on solid support.
The epitope recognized by A302-084A maps to a region between residue 2421 and 2471 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-084A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-084A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-084AOpen
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