Rabbit anti-Notch2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-084A maps to a region between residue 2421 and 2471 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Notch2 immobilized on solid support.
The epitope recognized by A302-084A-T maps to a region between residue 2421 and 2471 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).
Additional Product Information
Notch2 is a member of the Notch family of transmembrane receptors. The Notch gene was first reported as a drosophila gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch proteins mediate juxtacrine signals between adjacent cells and regulate cell fate determination during development. In mammals there are 4 Notch receptors (Notch 1-4). Like Notch1, Notch 2 is required for embryo viability. Although Notch1 and Notch 2 are functionally similar, they may not serve redundant functions in the cell.
Alternate Names
AGS2; HJCYS; hN2; neurogenic locus notch homolog protein 2; Notch 2; Notch homolog 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.