Bethyl Laboratories
Rabbit anti-Notch2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00539
TargetNotch2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Notch2 is a member of the Notch family of transmembrane receptors. The Notch gene was first reported as a drosophila gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch proteins mediate juxtacrine signals between adjacent cells and regulate cell fate determination during development. In mammals there are 4 Notch receptors (Notch 1-4). Like Notch1, Notch 2 is required for embryo viability. Although Notch1 and Notch 2 are functionally similar, they may not serve redundant functions in the cell.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2421 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetNotch2
Antigen Species
Human
Gene ID4853
Gene Symbol
NOTCH2
Gene Name
notch 2
Uniprot IDQ04721
Protein Name
Neurogenic locus notch homolog protein 2
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Notch2 immobilized on solid support.
The epitope recognized by IHC-00539 maps to a region between residue 2421 and 2471 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00539Open
Product DatasheetPDF · IHC-00539 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00539 · Lot 1 (current lot)Open
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