Bethyl Laboratories
Rabbit anti-NPAT Antibody Affinity Purified
Primary
Catalog #A302-772A
TargetNPAT
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
NPAT (nuclear protein of the ataxia telangiectasia mutated locus) is required for progression through the G1 and S phases of the cell cycle and for S phase entry. It activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. NPAT also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q14207].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1377 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNPAT
Antigen Species
Human
Gene ID4863
Gene Symbol
NPAT
Gene Name
nuclear protein, coactivator of histone transcription
Uniprot IDQ14207
Protein Name
Protein NPAT
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NPAT immobilized on solid support.
The epitope recognized by A302-772A maps to a region between residue 1377 and 1427 of human Nuclear Protein, Ataxia-Telangiectasia Locus using the numbering given in entry NP_002510.2 (GeneID 4863).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-772A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-772A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-772AOpen
Citations / Publications
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