Bethyl Laboratories
Rabbit anti-NPM1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00678
TargetNPM1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
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Product Description
Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06748].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 244 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetNPM1
Antigen Species
Human
Gene ID4869
Gene Symbol
NPM1
Gene Name
nucleophosmin 1
Uniprot IDP06748
Protein Name
Nucleophosmin
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NPM1 immobilized on solid support.
The epitope recognized by IHC-00678 maps to a region between residue 250 and 294 of human nucleophosmin using the numbering given in entry NP_002511.1 (GeneID 4869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00678Open
Product DatasheetPDF · IHC-00678 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00678 · Lot 1 (current lot)Open
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