Bethyl Laboratories
Rabbit anti-NS1-BP Antibody Affinity Purified
Primary
Catalog #A302-879A
TargetNS1-BP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
NS1-binding protein (NS1-BP) plays a role in cell division and in the dynamic organization of the actin skeleton by functioning as a stabilizer of actin filaments via its association with F-actin through Kelch repeats. NS1-BP may be a component of the cellular splicing machinery with a role in pre-mRNA splicing and may mediate the inhibition of splicing by NS/influenza virus NS1A protein. NS1-BP has also been found to function as modifier of the AHR/Aryl hydrocarbon receptor pathway by increasing the concentration of AHR available to activate transcription [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9Y6YO].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNS1-BP
Antigen Species
Human
Gene ID10625
Gene Symbol
IVNS1ABP
Gene Name
influenza virus NS1A binding protein
Uniprot IDQ9Y6Y0
Protein Name
Influenza virus NS1A-binding protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NS1-BP immobilized on solid support.
The epitope recognized by A302-879A maps to a region between residue 50 and 100 of human NS1-Binding Protein using the numbering given in entry NP_006460.2 (GeneID 10625).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-879A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-879A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-879AOpen
Citations / Publications
Services
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