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Primary Antibodies  

Rabbit anti-NSF Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NSF

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 500 and 550

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

N-ethylmaleimide-sensitive fusion protein (NSF) is required for vesicle-mediated transport. It catalyzes the fusion of transport vesicles within the Golgi cisternae. It is also required for transport from the endoplasmic reticulum to the Golgi stack. NSF seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46459].

4905

NSF

N-ethylmaleimide sensitive factor, vesicle fusing ATPase

P46459

Vesicle-fusing ATPase

Alternate Names

epididymis secretory sperm binding protein; NEM-sensitive fusion protein; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; SEC18; SKD2; vesicle-fusing ATPase; vesicular-fusion protein NSF

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.