Bethyl Laboratories
Rabbit anti-NUCKS Antibody Affinity Purified
Primary
Catalog #A301-508A
TargetNUCKS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 193 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNUCKS
Antigen Species
Human
Gene ID64710
Gene Symbol
NUCKS1
Gene Name
nuclear casein kinase and cyclin dependent kinase substrate 1
Uniprot IDQ9H1E3
Protein Name
Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUCKS immobilized on solid support.
The epitope recognized by A301-508A maps to a region between residue 147 and 197 of human nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate using the numbering given in entry NP_073568.2 (GeneID 64710).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-508A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-508A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-508AOpen
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