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Rabbit anti-NUF2 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
NUF2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 375 and 425
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to NUF2 immobilized on solid support.

The epitope recognized by A304-318A-T maps to a region between residue 375 to 425 of human Nuclear Fallout 2 using the numbering given in entry NP_663735.2 (GeneID 83540).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

NDC80 kinetochore complex component (NUF2) acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. NUF2 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BZD4].

83540

NUF2

NDC80 kinetochore complex component NUF2

Q9BZD4

Kinetochore protein Nuf2

Alternate Names

cancer/testis antigen 106, CDCA1, cell division cycle associated 1, cell division cycle-associated protein 1, CT106, hNuf2, hNuf2R, hsNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog, NUF2R

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.

Citations (1)