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Rabbit anti-NUMA Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
NUMA
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 900 and 950
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
40 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to NUMA immobilized on solid support.

The epitope recognized by A301-509A-T maps to a region between residue 900 and 950 of human nuclear mitotic apparatus protein 1 using the numbering given in entry NP_006176.2 (GeneID 4926).

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Gene & Protein Information

The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.

4926

NUMA1

nuclear mitotic apparatus protein 1

Q14980

Nuclear mitotic apparatus protein 1

Alternate Names

centrophilin stabilizes mitotic spindle in mitotic cells, NMP-22, nuclear matrix protein-22, Nuclear mitotic apparatus protein, nuclear mitotic apparatus protein 1, NUMA, NuMA protein, SP-H antigen, structural nuclear protein

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (1)