/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NUMA Antibody
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Primary Antibodies  

Rabbit anti-NUMA Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (3)
Target:

NUMA

Reactivity:

Human

Applications:

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 900 and 950

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.

4926

NUMA1

nuclear mitotic apparatus protein 1

Q14980

Nuclear mitotic apparatus protein 1

Alternate Names

centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; nuclear matrix protein-22; Nuclear mitotic apparatus protein; nuclear mitotic apparatus protein 1; NUMA; NuMA protein; SP-H antigen; structural nuclear protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.